Question: Merge multiple overlapping consensus sequences
0
gravatar for kspata
3 months ago by
kspata30
kspata30 wrote:

Hi All,

I have 10 consensus sequence files which have some overlapping regions between them. I would like to merge all of them to generate single consensus fasta file. I have looked into emboss merger but it takes only two input files. How can I achieve this? Which software should I use?

Thanks in advance!!!

assembly consensus • 199 views
ADD COMMENTlink written 3 months ago by kspata30
2
gravatar for genomax
3 months ago by
genomax55k
United States
genomax55k wrote:

Do a multiple sequence alignment (MSA) with all 10 and then export a consensus. Most MSA programs can be used for this.

ADD COMMENTlink written 3 months ago by genomax55k

Thanks @genomax!!! I used Clustal Omega to align the consensus sequences with each other. Downloaded the JalView results and obtained a consensus sequence.

ADD REPLYlink written 3 months ago by kspata30

Perfect. So all done then. I will move my comment to an answer that you can accept to provide closure to the thread.

ADD REPLYlink modified 3 months ago • written 3 months ago by genomax55k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1308 users visited in the last hour