Question: Merge multiple overlapping consensus sequences
0
gravatar for kspata
4 weeks ago by
kspata30
kspata30 wrote:

Hi All,

I have 10 consensus sequence files which have some overlapping regions between them. I would like to merge all of them to generate single consensus fasta file. I have looked into emboss merger but it takes only two input files. How can I achieve this? Which software should I use?

Thanks in advance!!!

assembly consensus • 104 views
ADD COMMENTlink written 4 weeks ago by kspata30
1
gravatar for genomax
4 weeks ago by
genomax52k
United States
genomax52k wrote:

Do a multiple sequence alignment (MSA) with all 10 and then export a consensus. Most MSA programs can be used for this.

ADD COMMENTlink written 4 weeks ago by genomax52k

Thanks @genomax!!! I used Clustal Omega to align the consensus sequences with each other. Downloaded the JalView results and obtained a consensus sequence.

ADD REPLYlink written 4 weeks ago by kspata30

Perfect. So all done then. I will move my comment to an answer that you can accept to provide closure to the thread.

ADD REPLYlink modified 4 weeks ago • written 4 weeks ago by genomax52k
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