Merge multiple overlapping consensus sequences
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3.3 years ago
kspata ▴ 70

Hi All,

I have 10 consensus sequence files which have some overlapping regions between them. I would like to merge all of them to generate single consensus fasta file. I have looked into emboss merger but it takes only two input files. How can I achieve this? Which software should I use?

Thanks in advance!!!

assembly consensus • 1.6k views
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3.3 years ago
GenoMax 107k

Do a multiple sequence alignment (MSA) with all 10 and then export a consensus. Most MSA programs can be used for this.

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Thanks @genomax!!! I used Clustal Omega to align the consensus sequences with each other. Downloaded the JalView results and obtained a consensus sequence.

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Perfect. So all done then. I will move my comment to an answer that you can accept to provide closure to the thread.

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