Question: Merge multiple overlapping consensus sequences
0
gravatar for kspata
7 months ago by
kspata40
kspata40 wrote:

Hi All,

I have 10 consensus sequence files which have some overlapping regions between them. I would like to merge all of them to generate single consensus fasta file. I have looked into emboss merger but it takes only two input files. How can I achieve this? Which software should I use?

Thanks in advance!!!

assembly consensus • 410 views
ADD COMMENTlink modified 4 months ago by RamRS20k • written 7 months ago by kspata40
2
gravatar for genomax
7 months ago by
genomax60k
United States
genomax60k wrote:

Do a multiple sequence alignment (MSA) with all 10 and then export a consensus. Most MSA programs can be used for this.

ADD COMMENTlink written 7 months ago by genomax60k

Thanks @genomax!!! I used Clustal Omega to align the consensus sequences with each other. Downloaded the JalView results and obtained a consensus sequence.

ADD REPLYlink written 7 months ago by kspata40

Perfect. So all done then. I will move my comment to an answer that you can accept to provide closure to the thread.

ADD REPLYlink modified 7 months ago • written 7 months ago by genomax60k
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