I am trying to do long read alignments for my assembled reads with ncbi protein database. I was trying to use LAST tool available here (http://last.cbrc.jp/doc/last-tutorial.html) using nr.gz database using the command
lastdb -cR01 nrdb nr.gz. However, it doesn't seem to work with
.gz format. Is there a way I can extract all sequences from
nr.gz into fasta format? Or if anyone has better suggestion on what I should be doing to perform this alignment? Thanks for your help in advance.