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6.3 years ago
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I am trying to do long read alignments for my assembled reads with ncbi protein database. I was trying to use LAST tool available here (http://last.cbrc.jp/doc/last-tutorial.html) using nr.gz database using the command lastdb -cR01 nrdb nr.gz
. However, it doesn't seem to work with .gz
format. Is there a way I can extract all sequences from nr.gz
into fasta format? Or if anyone has better suggestion on what I should be doing to perform this alignment? Thanks for your help in advance.
You will need to
gunzip nr.gz
to get the fasta format file. Based on the tutorial that is whatlastdb
needs.Thanks for the helpful comment.