The alignment with the whole human genome and my data, the sam files are genertaed but when I tried to use the samtools for conversion sam to bam facing problem of header. with the reference sequence also am not able to do it.
Any other way to do it?
[sam_header_line_parse] expected '@XY', got [@A00306:15:H3WG3DMXX:1:1101:14705:1000 2:N:0:TAGCTTAT] Hint: The header tags must be tab-separated. [sam_header_read2] 0 sequences loaded. [samopen] no @SQ lines in the header. [main_samview] random alignment retrieval only works for indexed BAM files.