Entering edit mode
5.7 years ago
rnaseqbias
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0
Hello. What is the cause of 3' bias in the polyA selection step in RNASeq library preparation? (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310221/figure/F6/) I have searched around on the internet but could not find the mechanism and cause explicitly explained. Is there a mathematical model for the bias?
Thank you.
Since poly-A tails are at the 3'-end of RNA molecules it is not surprising this happens.
Is the bias because of biochemical or mechanical reasons? If it is a purely biochemical process, then the 3' bias should also show up if ribo-depletion is used instead of polyA selection to get rid of ribosomal RNA.(But as far as I know this is not the case.) So is there a mechanical aspect to this where the polyA grabbing induces breakage?