Question: Differential expression analysis of normalized expression of RNA seq data
0
gravatar for Javad
14 months ago by
Javad60
Javad60 wrote:

Dear All,

I have a normalized expression table of RNA seq data. I normally use DESeq2 but it needs raw count table not normalized data. Which R package I can use to analyse a Normalised table?

Best,

rna-seq • 700 views
ADD COMMENTlink modified 14 months ago by Benn7.8k • written 14 months ago by Javad60
1

Normalized by what method? Are there replicates?

ADD REPLYlink modified 14 months ago • written 14 months ago by ATpoint24k
1

This post from the bioconductor support site maybe of help: https://support.bioconductor.org/p/56275/

ADD REPLYlink written 14 months ago by ejm32440
0
gravatar for Benn
14 months ago by
Benn7.8k
Netherlands
Benn7.8k wrote:

None. There is no package that is able to do (sound) statistical analysis of normalized RNAseq data. Get read count data, and do what you normally do with DEseq2 or limma voom, etc.

ADD COMMENTlink modified 14 months ago • written 14 months ago by Benn7.8k
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