Question: Combine microarray and RNA-seq gene expression for differentially expression analysis
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gravatar for landscape95
13 months ago by
landscape95160
landscape95160 wrote:

Hi everyone, I wonder whether we can combine the RNA-seq data with microarray gene expression data together to do the differential expression analysis or others signature scoring methods since as far as I know, microarray is based on image intensity and RNA-seq is based on the counts of mapped reads?

Your help is really appreciated.

deg rna-seq microarray • 634 views
ADD COMMENTlink modified 13 months ago • written 13 months ago by landscape95160
1

I wonder whether we can combine the RNA-seq data with microarray data

Question unclear. Do you mean you want to compare dataset A with condition A (using microarray) with dataset B with condition B (using RNA-seq)? Or do you mean that your condition A has both samples quantified using microarray and RNA-seq, and you want to compare that with condition B which also has both microarray and RNA-seq data.

ADD REPLYlink written 13 months ago by WouterDeCoster41k
0
gravatar for Kevin Blighe
13 months ago by
Kevin Blighe50k
Kevin Blighe50k wrote:

I feel that you should conduct the studies independently and then perform a meta-analysis of the results. Take a look at RankProd

ADD COMMENTlink written 13 months ago by Kevin Blighe50k
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