Hi guys, I have a question about how to define a gene to be expressed in RNA Seq analysis. Is it better to use log2(rpkm) or rpkm without log2 transformation to define a gene to be expressed? I know that the log is only a transformation so that log2(rpkm) will let to consider as expressed a gene with a higher rpkm with respect to the simple rpkm. However I think that rpkm alone leads to consider as expressed genes that have too few reads.
Some ideas about?
Thank you in advance