Apologies for this basic question but I can not find a simple answer
I want to sequence several samples of mRNA paired end 2x150 and for each sample I want 100 million reads (100 million for forward and 100 million for reverse)
the supplier proposes to sequence 3 samples per lane of flow cell of the Hiseq 4000 technology. So what I understood 3 samples x 200 million reads (100 million for forward and 100 million for reverse) = 600 million reads per lane
but when I looked for the performances Illumina Hiseq 4000 I found this technology can generate 312000000 Clusters per lane only. How can this technology generate 600 million reads per lane ??
Would it be possible to give me a simple answer Thank you in advance