I'm study the Alternative splicing events of RNA-seq. I saw a artical, which said:
It was worth mentioning that some splicing transcripts of a certain gene were diﬀerentially expressed in diﬀerent libraries. We called these genes diﬀerentially expressed transcripts (DETs). And then, author compulated the expression (FPKM) of all splice transcripts of (DETs)
Firgure.1 part of the Splicing transcripts and gene structures, becaues of the size of picture, I just pasted a part of the picture.
Figure.2 The FPKM of splice transcripts.
I don't know how to compulate the FPKM of splice transcripts, or which software can calculate the values. If possiable, I also want to know how to draw the pictures like Figure. 1