I am working on an RNA-Seq project. We just got back some sequencing and I had a couple questions about it. I figure someone here might be able to help me out a bit.
So I think the sequencing doesn't look too bad. I don't really like trimming RNA-seq data if I don't have to, but I attached images of my FASTQC results.
The "Per tile sequence quality" makes me think that I may need to trim that data a bit? What do you think? I am also curious what people think about the "Adaptor content"? The read length for this project is 150 bp. Do you think those are just reads started to end at ~60 bp instead of the full 150 bp?
Let me know if you see anything else that really stands out for you. Again, I would prefer to do as little amount of trimming as possible.. if I even have to.