I want to use STAR to align my reads to a small reference genome (~12.500 UTRs). Although I have created a .fa-file of my library STAR is not able to generate a genome, as it is unable to read the file. I was wondering if anyone else has experienced the same problem? Or whether anyone has a solution to this problem?
Best, Bram Verhagen
./STAR --runMode genomeGenerate --genomeDir home/hub_tanenbaum/bverhagen/star-genome/ --genomeFastaFiles home/hub_tanenbaum/bverhagen/star-genome/sequence.fa