Question: What's your preferred pathway enrichment analysis tool after DEG analysis and why?
2
gravatar for unawaz
8 days ago by
unawaz40
Australia
unawaz40 wrote:

So firstly, I'm completely aware that this type of question has been asked multiple times (I know this since I've been scrolling over these type of questions for the past 2 days), but I'm actually more interested in knowing the reasons as to why some people prefer

I've performed differential expression analysis using DESeq2 and I want to see which Gene ontology terms, KEGG pathway terms etc are enriched in my data set. I've initially tried using clusterProfileR, but I keep getting 3 enriched terms for all my differentially expressed genes using enrichGO(). I also know that some input in clusterProfileR requires you to put logFC values, so I wasn't sure if that was for ALL the genes analysed, or just the differentially expressed genes.

I've also used goseq but my main issue with that is the GO terms are too broad.

I also only have about 300 DEGs, so I'm not really sure if this sort of analysis is best performed when you have a myriad of DEGs, or can be done with a small number.

Anyway, looking forward to hearing people's responses :)

rna-seq gene ontology • 175 views
ADD COMMENTlink modified 8 days ago by Jean-Karim Heriche17k • written 8 days ago by unawaz40
3
gravatar for i.sudbery
8 days ago by
i.sudbery3.1k
Sheffield, UK
i.sudbery3.1k wrote:

I'm a big user of GOseq, which allows to control for the fact that longer and more highly expressed genes are more likely to be found to be differential, if long or highly expressed genes are not evenly distributed between pathways/categories, then this can bias your enriched pathways.

I also like the GSEA-like algorithms, because you do not have to set an artificial limit on what you consider significant. The version of this where you rank on significance suffers from the same gene-length bias that traditional GO tests suffer, but this may be lessened by ranking on some suitably strunken logFC metric. cameraPR from limma is a good example of this.

Finally we've used SPIA before, which is a pathway enrichment tool that takes the topology of the network into account. Its a great idea, let down by the quality of the pathway annotations it runs on.

ADD COMMENTlink written 8 days ago by i.sudbery3.1k
1
gravatar for Devon Ryan
8 days ago by
Devon Ryan87k
Freiburg, Germany
Devon Ryan87k wrote:

Essentially every free tool is using the same set of databases and quite similar algorithms (there's a smallish set to choose from with a few tweaks here and there), so it's unsurprising that you get similar results regardless of which tool you use. To be frank, if you want different results you need to use a different database. We're pretty happy with IPA in this regard. It can be rather pricey, but if you can go in with multiple labs on a license then it becomes more feasible.

ADD COMMENTlink written 8 days ago by Devon Ryan87k
0
gravatar for EagleEye
8 days ago by
EagleEye6.1k
Sweden
EagleEye6.1k wrote:

I use Gene Set Clustering based on Functional annotation (GeneSCF)

ADD COMMENTlink written 8 days ago by EagleEye6.1k
0
gravatar for Jean-Karim Heriche
8 days ago by
EMBL Heidelberg, Germany
Jean-Karim Heriche17k wrote:

An alternative approach: Finding New Order in Biological Functions from the Network Structure of Gene Annotations

ADD COMMENTlink written 8 days ago by Jean-Karim Heriche17k
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