I use fast-x toolkit for quality filtering of my data. I get an error when running the command
fastq_quality_filter -v -Q 64 -q 25 -p 75 -i sra_data.fastq -o sample_filtered.fastq fastq_quality_filter: Invalid quality score value (char '.' ord 46 quality value -18) on line 4
I need a moderate threshold of average quality>25. please, can anyone suggest a possible solution for this?