Hi, I have aligned paired end RNA-Seq data and have the BAM file. I want to get the aligned reads using samtools. My command is : samtools view -b -F 4 filename.bam > mapped_out.bam Is it right for the paired end data? Also, when I am using the command, the generated file is found to be bigger than the input file. I would be grateful if anyone can help me to understand this.
Question: Extraction of aligned read using samtools
8 days ago by
Neu • 10
Neu • 10 wrote:
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