Hi, I have aligned paired end RNA-Seq data and have the BAM file. I want to get the aligned reads using samtools. My command is : samtools view -b -F 4 filename.bam > mapped_out.bam Is it right for the paired end data? Also, when I am using the command, the generated file is found to be bigger than the input file. I would be grateful if anyone can help me to understand this.
Question: Extraction of aligned read using samtools
4 months ago by
Neu • 10
Neu • 10 wrote:
ADD COMMENT • link •
Please log in to add an answer.
Powered by Biostar version 2.3.0
Traffic: 1604 users visited in the last hour