Question: How to create local database for doing BLAST
0
gravatar for Mehar Imran Zafar
13 months ago by
Virtual University of Pakistan
Mehar Imran Zafar 10 wrote:

hello everyone please guide me about how I can create a local database for identified genes by BLAST search against the EST sequence

how I can download EST Sequence of the respective plant species(eg Oryza sativa) in the local database and how I can perform BLAST against this EST sequence via identified genes

ADD COMMENTlink modified 13 months ago by genomax80k • written 13 months ago by Mehar Imran Zafar 10

You can take a look at E-utilities

ADD REPLYlink written 13 months ago by Bastien Hervé4.5k

Or follow some tutorials which can be found on google, I found for example here one.

ADD REPLYlink written 13 months ago by Benn7.9k
1
gravatar for genomax
13 months ago by
genomax80k
United States
genomax80k wrote:

Information you need to create local blast databases is available in this NCBI blast handbook.

ADD COMMENTlink written 13 months ago by genomax80k
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