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Question: How to create local database for doing BLAST
0
gravatar for Mehar Imran Zafar
9 months ago by
Mehar Imran Zafar • 10
Virtual University of Pakistan
Mehar Imran Zafar • 10 wrote:

hello everyone please guide me about how I can create a local database for identified genes by BLAST search against the EST sequence

how I can download EST Sequence of the respective plant species(eg Oryza sativa) in the local database and how I can perform BLAST against this EST sequence via identified genes

sequencing alignment sequence genome gene • 336 views
ADD COMMENT • link •
modified 9 months ago by genomax ♦ 75k • written 9 months ago by Mehar Imran Zafar • 10

You can take a look at E-utilities

ADD REPLY • link written 9 months ago by Bastien Hervé ♦ 4.5k

Or follow some tutorials which can be found on google, I found for example here one.

ADD REPLY • link written 9 months ago by Benn ♦ 7.9k
1
gravatar for genomax
9 months ago by
genomax ♦ 75k
United States
genomax ♦ 75k wrote:

Information you need to create local blast databases is available in this NCBI blast handbook.
ADD COMMENT • link written 9 months ago by genomax ♦ 75k
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