Question: How to find transcription factor downstream binding
0
gravatar for aw_gibbs
15 days ago by
aw_gibbs0
aw_gibbs0 wrote:

Hi all,

I would like to find out what the mouse Stat3 transcription factor binds to and the full signalling pathway(s) that it is involved in.

So far, I have only managed to find a signalling pathway that ends in Stat3, and subsequently genes that bind to Stat3.

I have tried JASPAR, but the website is down. Does anyone have any suggestions?

Thanks in advance!

Alex

rna-seq gene • 84 views
ADD COMMENTlink modified 12 days ago by chris86250 • written 15 days ago by aw_gibbs0
2

If you want actual binding data, search for Stat3 ChIP-seq on GEO/NCBI. JASPAR is just a database for motifs but a motif presence does not imply binding.

ADD REPLYlink written 12 days ago by ATpoint15k
2

Well I would say TF (for e.g stat3) binding and its target genes would be cell type specific. you may have in general stat3 binding motif and known putative targets, I am not sure about your objectives, however if you are interested in some type regulation in particular cell type, you may have to look for ChIPseq data for that particular TF.

ADD REPLYlink modified 12 days ago • written 12 days ago by Prakash920
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