Tool for visualisation of blast results
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4.8 years ago
s.m.frampton ▴ 20

Hello,

Is there a tool that provides a nice visualisation of the homology between 3 genes? Furthermore, I also really need to be able to see where the exons are within the figure to see which exons the genes share...

I have three homologous genes from a complex region of the human genome whereby one of them is a fusion of the other two (gene 1). I am trying to learn the technical limitations of short- and long-read sequencing when studying this region so need an understanding of where the reads have originated from and where they are likely to incorrectly map to.

I'm interested in a tool that generates something like the image attached (made with Kablammo) but with some tweaks. Kablammo only creates figures of homology between 2 genes and adjusts the size so they all look the same length, plus information about exons and introns is lost. I have made the attached figure by swapping which gene is the query/subject and attaching my own labels in PowerPoint.

Perhaps within the evolutionary genomics field there is a neat tool that can display all this?

Many thanks in advance! enter image description here

alignment homology RNA-Seq evolutionary genomics • 4.4k views
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maybe CoGe tool GEvo (https://genomevolution.org/wiki/index.php/GEvo ) could work or worth taking a look at.

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4.8 years ago
Asaf 10k

Take a look at genoplotR, it might need some R coding though.

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4.8 years ago
Joe 21k

You can take a look at this thread I've been curating Interesting/useful/beautiful bio sequence viz tools

Not all of those suggestions will fit the bill, but EasyFig probably would.

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