i have tumor.fasta file and i want to mapping it to reference Only TP53 region
How can i ?
By getting TP53 region sequence you are interested in as fasta format file. Followed by using blat (http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/blat/ ) to do alignment of your tumor.fasta to the reference.
What does tumor.fasta contain?