Hi everybody I want to merge reads (reverse and forward) so i use seqPrep with this command :
SeqPrep -f ERR770964_1.fastq -r /ERR770964_2.fastq -1 forward.fastq -2 reverse.fastq -s merged_file.fastq
, i got this result
Pairs Processed: 0
Pairs Merged: 1066735
Pairs With Adapters: 12980
Pairs Discarded: 6788
but the output files is not readable ,when i open the merged file i get somthing like this
▒▒ݒ▒J▒-v▒Oqn▒̞▒ĿdI▒▒▒#'▒8▒ ▒▒▒▒a,▒*▒gFs"\▒w▒J▒(▒▒▒▒▒▒▒0▒ƾ▒▒▒O▒▒▒▒▒=▒▒▒p+▒▒▒▒[▒▒▒▒v▒▒1▒Bsk▒▒▒▒▒▒▒▒▒_▒c▒r▒▒▒E▒E▒▒_r&▒(▒▒#▒▒`▒▒~▒▒_I▒p▒;lK_B▒#fMr<▒▒_▒▒Jr▒▒▒bJ9▒^▒▒Ɩ▒▒C]o▒▒zr▒▒▒▒▒&w▒&▒7▒▒▒▒▒▒▒)▒▒>▒▒H▒#[▒k▒▒:~▒▒÷)▒ld#▒▒▒ɹ▒▒▒t[▒_|▒▒▒iN)▒q0▒c▒▒▒O~▒▒▒▒<▒▒▒6C▒Oh▒▒▒▒▒\UU▒▒fZ{▒▒f[▒▒VU{▒▒C▒▒▒+▒▒iJ▒g▒tk▒&▒▒7▒hˇ▒▒▒~▒l)▒▒▒▒O▒▒"u&Xx▒E%Y▒1▒Bq$▒▒5▒.6▒S▒▒Q#▒b▒▒▒▒\▒+▒Gr▒"▒▒▒XV{&▒b▒▒0ڜ▒▒UW▒▒Mߑ▒▒Mi▒v▒▒tLN▒"_▒6[▒s▒▒y▒▒▒vMӖ▒'eG=▒6/vC▒▒▒▒▒-▒4▒▒�▒{▒▒▒ۆBU▒▒>▒▒▒▒▒?i▒▒}▒oT▒▒Y▒'▒▒▒▒/~h▒|▒b▒▒▒D▒▒gWW▒▒T▒▒T▒D▒# ▒▒j▒]▒▒▒Ӕ▒▒l▒▒;▒▒o~▒ please can you help me because i m begeniinng in this field thanks
Even though your file names say
.fastq
it looks like your files may be compressed/gzipped. What do you get if you dofile ERR770964_1.fastq
andfile ERR770964_2.fastq
?My files is not compressed , it's fastq file i don't indrstand when i have problem
I asked you to provide output for the two commands above. Without that there is not much we can say.
If you are Linux user try zcat merged_file.fastq you can decompress via gunzip merged_file.fastq, then open it