I want to merge my pair-end reads into one single reads with about 50 bp overlap. However, my fastq file is Illumina-1.8 Phred+33 format, so the maximum score is 41 (J). I tried NGmerge for merging but it can not expand to score 41 with -u 41 and editing the qual_profile. Is there any other method available to merge this kind of data? Need your help. Thank you!
Question: how to merge pair-end reads with maximum score 41 (J)
10 weeks ago by
tiantai_wing • 0
tiantai_wing • 0 wrote:
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