Question: TCGA survival analysis using >100 genes
1
gravatar for veronico
14 months ago by
veronico60
veronico60 wrote:

Hi everyone,

I hope everyone is doing well!

I was wondering if anyone knew how to do survival analysis by using a panel of >100 genes? I have a list of genes I am interested in (>100 genes) and I want to find these list of genes that are down regulated from RNA seq data from TCGA (preferably Breast Cancer), please?

I was using this method, that was amazingly made by TriS: Survival analysis of TCGA patients integrating gene expression (RNASeq) data

However, I am unsure on how to 1) find only downregulared genes and 2) do survival analysis pertaining to >100 genes.

My apologies for the newb question. I am new to R. I got TriS' method to work for genes altered, but I am interested in a different question.

Thank you!

survival rna-seq tcga many • 388 views
ADD COMMENTlink modified 14 months ago by h.mon31k • written 14 months ago by veronico60

With R loop, you can make 60,000 survival analysis for each RNA-seq gene.

ADD REPLYlink written 14 months ago by Shicheng Guo8.4k
1

Skip loops, use apply instead.

ADD REPLYlink written 14 months ago by RamRS30k
1

Ok, thank you. I'll look into apply

ADD REPLYlink written 14 months ago by veronico60
1

for each gene? My apologies I am not following. Would this work if I have a panel of a >100 genes that I want to investigate together?

ADD REPLYlink written 14 months ago by veronico60

Sure. I think you should make a better definition for. What exactly you want to do. "do survival analysis by using a panel of >100 genes" is not so clear.

ADD REPLYlink written 14 months ago by Shicheng Guo8.4k
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