In my transgenic rice (over-expression gene A), there are several lines with different expression levels, I've measured phenotype XX for N individuals in each line. So how can I make the conclusion whether A has effect on phenotype XX ?
For example, I have 10 lines with diverse expression levels of gene A, each line has 10 individuals, I measured each individuals in each line. I can make a boxplot to see this, but how can I get the significance extent. Or just get the mean value of phenotype in each line, then do pearson correlation between phenotype mean in each line and the expression levels?
Thank you very much!