Trinity showing up fastq entry error ?

Question

[sunnykevin97]

HI

I had few RNA_Seq datasets to generate denovo assemblies from them

Read length - 150 PE library RAM- 10GB

I performed preprocessing ( N + adapter trim) and subjected to Trintiy

always I'm getting this error ? (I tried with other datasets as well, facing same error)

How do i rectify this error ? (Is the error due to insufficient computational power) suggestions please!

Thread 8 terminated abnormally: Error, Error, fastQ entry doesn't have 4 lines: @NB501461:5:H2F2CBGXY:1:13111:26148:17274 2:N:0:TTAGGC

In the output directory, it generated only these (for all the datasets) chrysalis insilico_read_normalization Trinity.timing

Answer 1

[GenoMax]

It appears that your fastq file is either originally corrupted or got corrupted during the initial processing. You can check both files using the program linked here: C: Viewing and editing FASTQ files