design primers across the target sequence
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2.7 years ago
APJ ▴ 40

Hello,

I have a short target DNA sequence (40 nucleotides in length) in the given transcript DNA sequence. I wanted to design the PCR primers(reverse/forward) across this region. Is there any solution to do in biopython. If not, how can one do such analysis on NCBI-primer blast tool? Any suggestions or comments please? Thank you!

ncbi primer design primer3 biopython SeqIO • 566 views
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https://www.ncbi.nlm.nih.gov/guide/howto/design-pcr-primers/

Check YouTube for additional tutorials if you learn visually.

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I suggest you start by reading the documentation of PrimerBLAST.

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