Entering edit mode
4.2 years ago
APJ
▴
40
Hello,
I have a short target DNA sequence (40 nucleotides in length) in the given transcript DNA sequence. I wanted to design the PCR primers(reverse/forward) across this region. Is there any solution to do in biopython. If not, how can one do such analysis on NCBI-primer blast tool? Any suggestions or comments please? Thank you!
https://www.ncbi.nlm.nih.gov/guide/howto/design-pcr-primers/
Check YouTube for additional tutorials if you learn visually.
I suggest you start by reading the documentation of PrimerBLAST.
If it helps? - A: primer design to discriminate sequences in intron-exon MSA