I am studying bioinformatics, non-biological major.
I encountered some problems in reproducing the results of a paper. The DOI of this paper is 10.1007/s10753-019-01060-8.
This paper analyzes circRNA, miRNA, and mRNA of rats, but it uses whole genome sequencing. I tried to deal with it myself but failed.
I downloaded the reference sequence and annotation file of the rat, the URL is this , but I don’t know if it is correct?
I searched a lot of data on whole genome sequencing processing on the Internet, but most of them were doing mutation analysis.
I want to know how to get RNA data from whole genome sequencing data.