Tutorial: RNASeq tutorial for gene differential expression analysis
gravatar for Thind Amarinder
16 days ago by
Thind Amarinder30 wrote:


This tutorial is based on Bioconductor packages, RNAseq gene differential expression analysis, including filtering, preprocessing, visualization, clustering, and Enrichment. In case you have any queries or questions, please feel free to ask or correct. I hope it will be useful for new Bioconductor users.

Required data files You should have a raw count and annotation/metadata file for running this analysis. Raw count files are usually obtained from tools such as feature count, Rsem, etc.

Bioconductor packages to be installed



biomaRt (Very useful for gene filtering and annotations)

PCAtools (PCA detailed analysis)

ReactomePA (enrichment analysis)

Note: PCA and Enrichment analysis is based on Deseq2. However, users may be interested in considering only those genes that are commonly differentially expressed between DEseq2 and EdgeR.

R script and further instructions of the tutorial are available here.


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