Question: Turn Off Blast Search On Reverse Complement Strand In Blastn
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Zhaorong • 1.3k wrote:
I have a quick question: How can I turn off search on reverse complement strand of my query nucleotide sequence in blastn?
For example, I don't want 'GUAAAGCCAAAUCUUCGGUUA' to be a hit when I use 'UAACCGAAGAUUUGGCUUUAC' as the query.
Maybe I missed it when I read the man page, but I really appreciate it if someone can point out the parameter I should use.
Thanks!
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yasopi9568 • 0
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10.9 years ago by
Zhaorong • 1.3k
I think you should someone take care of the target or subject strandedness as well. You should post - filter the results based on the alignment location I guess in query and subject/ target