Entering edit mode
13.1 years ago
Zhaorong ★ 1.4k
I have a quick question: How can I turn off search on reverse complement strand of my query nucleotide sequence in blastn?
For example, I don't want 'GUAAAGCCAAAUCUUCGGUUA' to be a hit when I use 'UAACCGAAGAUUUGGCUUUAC' as the query.
Maybe I missed it when I read the man page, but I really appreciate it if someone can point out the parameter I should use.
I think you should someone take care of the target or subject strandedness as well. You should post - filter the results based on the alignment location I guess in query and subject/ target