Please could you clarify for me the different metrics associated with sequencing data. What is the difference between depth and coverage.
I have seen a question with this title closed already because it is was classed as an exact duplicate to this question. I have read that question and thought I understood the answer. However i have just seen another question on this forum here which leads me to think that depth and coverage are different things as the people posing the question have given different quality metrics for depth and coverage
e.g. depth (ex min. 50X)
e.g. coverage (ex min 90% per sample/per region)
e.g. quality score (ex. 90% of all sites [depth > 50X and Q20])
so in order to stop this question being closed as a duplicate, perhaps my question should be phrased as, please can you explain the terms depth/coverage/quality score given in the question here
Thanks a lot
Hummm... Another definition question! I think we should set a standard answer for this one. It will certainly reaper sooner or later. Where's the wiki?
+1 For the idea to document standard questions and answers on a wiki. And it would be great to make slides based on that as well.