I have histone chip-seq data for 3 developmental stages. I want to find out the differential histone acetyl marks between these 3 stages. I've been trying to work with DiffBind but unfortunately I have no replicates so I cant use that. Does anyone have any suggestions as to how I can do this without replicates?Are there any tools out there or packages in R that deal with this problem. I know there is Bedtools but anything else?
Thanks a lot!!!!