Question: Illumina Read Quality
0
gravatar for hellbio
6.6 years ago by
hellbio410
hellbio410 wrote:

Hi all,

I am new to NGS and i have a very naive question.

Illumina reports the score of each base in the read as a phred score. But, how is the total read quality calculated?Is it the sum of scores of all the bases in the read? Any comments are valuable.

illumina ngs • 3.6k views
ADD COMMENTlink modified 6.6 years ago by Josh Herr5.7k • written 6.6 years ago by hellbio410

Similar one was discussed here

ADD REPLYlink modified 14 months ago by RamRS26k • written 6.6 years ago by always_learning1.0k
0
gravatar for Josh Herr
6.6 years ago by
Josh Herr5.7k
University of Nebraska
Josh Herr5.7k wrote:

There are a lot questions here focused on read quality so your question is redundant -- you can find other questions relating to your topic by using the custom search.

I would also recommend this page -- FASTQ format and read quality -- to provide you some background on how the Phred quality score is calculated and what each character means.

ADD COMMENTlink modified 14 months ago by RamRS26k • written 6.6 years ago by Josh Herr5.7k
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