Question: Why I Am Getting This Error " No Alias Or Index File Found For ..... Database"
0
gravatar for bioinformaticswelkinakash
5.1 years ago by

I format my yeast.aa amino acid with this command

makeblastdb -dbtype prot -in yeast.aa -hash_index

it created several files then i run this query command with blastp

blastp -query reptiles.fasta -db yeast

which throws error saying

no alias or index file found for ......

please resolve my issue

actually i want to align two seq on my machine using standaloneblast+

• 10k views
ADD COMMENTlink modified 5.1 years ago by Neilfws48k • written 5.1 years ago by bioinformaticswelkinakash0

It doesn't find your database. So where is it?

ADD REPLYlink written 5.1 years ago by Michael Dondrup46k

sir i have placed yeast.aa in same folder where after executing makeblast all file is also kept , ifact all file in same folder from all exe to db files

ADD REPLYlink written 5.1 years ago by bioinformaticswelkinakash0

try blastp -query reptiles.fasta -db yeast.aa if that doesn't work locate the full path to yeast.aa and try that: blastp -query reptiles.fasta -db /path/to/yeast.aa

ADD REPLYlink modified 5.1 years ago • written 5.1 years ago by Michael Dondrup46k
2
gravatar for 5heikki
5.1 years ago by
5heikki8.4k
Finland
5heikki8.4k wrote:

If you don't specify a name for your db, then it will be exactly the same as the input file, i.e. in this case "yeast.aa". The files in your directory are called yeast.aa.nhd, yeast.aa.nhi, etc., aren't they?

ADD COMMENTlink written 5.1 years ago by 5heikki8.4k

yes they are. but why I am getting this error message " No alias or index file found for protein database [yeast] in search path [c::\user\welkin_akash\Desktop\Job portal\vvk sir\bin; ;]

how to create that index file

ADD REPLYlink written 5.1 years ago by bioinformaticswelkinakash0
1

The error is because in your blastp command line you call the database "yeast", when it should be called "yeast.aa".

Alternatively, use "-out yeast" in the makeblastdb command line, to name the database "yeast".

ADD REPLYlink written 5.1 years ago by Neilfws48k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1231 users visited in the last hour