I'm doing de novo transcriptomics where no genome and transcriptome is available.
In the study, I've used three time points i.e., control(0 hour stress), 3 hour stress and 6 hour stress treatment.
For the RNA quantification, I'm using Kallisto tool for which I need to generate an index file. Which fastq data or file I should use for the generation of index which I can used for the quantification purpose.
Thank you in advance