Entering edit mode
2.7 years ago
Sabeen
▴
30
Hello everyone,
I need some info regarding how to get percentage of REF and ALT nucleotide sequence in my data.
I am using gatk and currently not getting REF and ALT percentages . the command i am using for the gatk vcf file is following
gatk --java-options "-Xmx8G" HaplotypeCaller -R /Users/sabeen/NGS/singleread/1/hg19.fa -I /Users/sabeen/NGS/singleread/1/reads-with-RG.bam -O /Users/sabeen/NGS/singleread/1/output_final.vcf
(how I need the info for for the reference and alternative nucleotide in percentages as follows.)
Ref
G(52%) A(32%)
Alt
A(48%) T(68%)
Really appreciate some guidance .
Thanks.
Bonjour a tous, j'ai le même problème j'aimerai avoir Le pourcentage des ref et alt dans un fichier VCF de la forme :
Bien Merci. je suis nouveau dans ce domaine.
This is an english forum. Merci.
please excuse me if I expressed myself in French
have a look at the FORMAT/AD in the genotypes.