hello everyone. I need to design primers for full length gene amplification of Gossipium hirsutum followed by transformation in local cotton variety. the sequences are retrieved from NCBI (XM_041098083.1, XM_041116887.1 and have many more like these).
- How would I know which gene is already full length, any indication or pointer that will help to find.
- Please guide do I need to start designing primer from the first ATG to the last stop codon in sequence. Or I need to design primers from the start codon of my desired protein present in the sequence.