I am interested in generating realistic rna-seq. Does anyone know tools that use 1) reference transcript and 2) isoform expression as input and then genreate realistic rna-seq? By meaning reference transcript, I am referring to this here (https://www.ncbi.nlm.nih.gov/projects/genome/guide/human/index.shtml).
Imagine the top blue bar is the reference transcript. Would there be such tools or ways to generate the realistic rna-seq reads (the small bar with red head and tail)?
Any read simulation tool should work.
randomreads.shfrom BBMap suite are a couple of examples.
Hi Thanks! I am trying to understand this link. Are you saying using reference transcripts as an input, I can get rna-seq data from this tool?