Entering edit mode
13 months ago
eggrandio
▴
40
Hello,
We have received the results of a library sequencing and we have noticed that the sequence quality drops at the 3' end of one of the pairs:
Moreover, there seems to be a biased base distribution too:
What could be the case? We still have not performed manual filtering of sequences, but I wanted to know what could be causing this.
Anyways, the sequence quality seems somewhat good.
Best,
Why Does The Base Quality Drop Towards The End For Illumina Reads ?
But shouldn't quality drop at both 3'ends and not just on the left one?
This may be one of those things that just happens to be a one-time thing with whatever was going on with this run. Was this sample pooled with others you did not prepare/own?
What machine? (Nextseq? Novaseq?) what library prep method?