Question: Insert Size And Fragment Size ?
10
gravatar for Nicolas Rosewick
3.5 years ago by
Belgium, Brussels, Université Libre de Bruxelles / Université de Liège
Nicolas Rosewick5.3k wrote:

Hi,

Simple question but I'm a little bit confused due to different description I read. So what is the difference between insert size and fragment size in DNA-Seq ( or RNA-Seq ) ?

Thanks

• 18k views
ADD COMMENTlink modified 22 months ago by -_-470 • written 3.5 years ago by Nicolas Rosewick5.3k
30
gravatar for -_-
22 months ago by
-_-470
Canada
-_-470 wrote:

See the above figure ( from What is the difference between a Read and a Fragment in RNA-seq? )

ADD COMMENTlink modified 7 months ago • written 22 months ago by -_-470

@-_- Link is broken. Btw, thanks for the picture, it tells enough!

ADD REPLYlink modified 13 months ago • written 13 months ago by manetsus40
5
gravatar for murali
3.5 years ago by
murali80
Germany
murali80 wrote:

The insertsize dependent on sequencing platform.

Insert_size = the sequence betwwn adapters

Fragment_size= Insert_size + Adapters

The link will clarify your ambuguity.

ADD COMMENTlink written 3.5 years ago by murali80
4
gravatar for lelle
3.5 years ago by
lelle740
Berlin
lelle740 wrote:

I guess that depends on the person who is using it.

I would say (for Illumina): Fragment size is the size of the actual fragments that you put into the sequencer (including adapter sequences). Insert size is the length of the DNA (or RNA) that you want to sequence and that is "inserted" between the adapters (so adapters excluded).

BUT fragment size yould also be the size of the fragments after shearing which would be insert size by my definition above.

ADD COMMENTlink written 3.5 years ago by lelle740
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