Question: Insert Size And Fragment Size ?
11
gravatar for Nicolas Rosewick
4.2 years ago by
Belgium, Brussels
Nicolas Rosewick6.3k wrote:

Hi,

Simple question but I'm a little bit confused due to different description I read. So what is the difference between insert size and fragment size in DNA-Seq ( or RNA-Seq ) ?

Thanks

• 24k views
ADD COMMENTlink modified 2.5 years ago by -_-590 • written 4.2 years ago by Nicolas Rosewick6.3k
34
gravatar for -_-
2.5 years ago by
-_-590
Canada
-_-590 wrote:

See the above figure ( from What is the difference between a Read and a Fragment in RNA-seq? )

ADD COMMENTlink modified 15 months ago • written 2.5 years ago by -_-590

@-_- Link is broken. Btw, thanks for the picture, it tells enough!

ADD REPLYlink modified 21 months ago • written 21 months ago by manetsus40
5
gravatar for murali
4.2 years ago by
murali90
Germany
murali90 wrote:

The insertsize dependent on sequencing platform.

Insert_size = the sequence betwwn adapters

Fragment_size= Insert_size + Adapters

The link will clarify your ambuguity.

ADD COMMENTlink written 4.2 years ago by murali90
4
gravatar for lelle
4.2 years ago by
lelle740
Berlin
lelle740 wrote:

I guess that depends on the person who is using it.

I would say (for Illumina): Fragment size is the size of the actual fragments that you put into the sequencer (including adapter sequences). Insert size is the length of the DNA (or RNA) that you want to sequence and that is "inserted" between the adapters (so adapters excluded).

BUT fragment size yould also be the size of the fragments after shearing which would be insert size by my definition above.

ADD COMMENTlink written 4.2 years ago by lelle740
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