Forum:How can a graduate student start offering bioinformatics pipeline development as freelance work for wet lab researchers?
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11 days ago
DdogBoss ▴ 40

I’m a graduate student improving my skills in building computational pipelines for genomic data. I’d like to start freelancing to build a portfolio and gain experience working with real wet lab data, and have the intuition that anybody working with genomic data can make use of computational pipelines.

So far, I’ve reached out to a few PhD contacts in wet labs at other universities, but I’m wondering what other avenues might be effective for finding projects.

Do researchers advertise small-scale bioinformatics help within universities (e.g., through listservs or departmental boards)?

Have others had success offering services through general freelance platforms (UpWork, Fiverr) or domain-specific ones like KolabTree?

I’d appreciate any advice from people who’ve successfully connected with wet lab scientists for freelance or collaborative projects.

snakemake career-advice nextflow • 1.5k views
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Ask yourself what your long-term goal is. We made the experience over the last decade that you can go two ways: You give data to someone who might technically be qualified but has no expert knowledge in the specific field or domain that you're working on, say a particular niche in immunity, or a specific cancer entity, and you will get lists and plots of results. Basically unreviewed or partially reviewed due to the lack of expert biological knowledge. This has limited value, as details often matter, and obtaining final publishable results that really drive a project forward requires constant discussion, redefining questions and adjusting analysis accordingly. For the projects we published, or are about to publish soon, there is no! way a freelancer could have contributed pretty much anything, other than do the preprocessing, such as aligning data, do basic QC etc. I need to agree that our field and what we do is very specialized, so it might not be representative, but we always get most meaningful results if we train the lead biologist to become at least a basic ChatGPT-assistet analyst, so they can hands-on see data, decide what makes sense and what doesn't and don't hit the wall finding a common language with an analyst from outside of the field. Some might argue that "blind" analysis from outside of the field comes with the benefit of being unbiased, but it suffers greatly from lack of expertise to know what does not make sense, and since biology is so vastly complex, the latter is the far greater risk ... as said, all in our experience, others might disagree, especially if analysis is very general, so you just do RNA-seq and all you need is a list of differential genes. Anyone can do this basically, AI-assistent if needed, today more than ever.

So what is your longterm plan? Do you want to become a pure service provider, or do you want to drive a project hands-on? If the latter, consider applying for a PhD, at best in a lab that allows to do both wetlab and computational work, as this is what the modern biologist needs.

That having said, what is your long-term goal?

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The freelancing idea would be a way to get experience, while simultaneously providing other people with a service that they may need.

If I were to take the freelancing idea more seriously, it would be to give people tools to have more reproducible analyses. Rather than relying on a bunch of glued together scripts from 20 years ago, they have a modern analysis pipeline that they can rerun reliably and that will be standardized among other labs.

Of course I would need to make sure that there would be a good handoff with the lead biologist or whoever else will be using the pipeline. That means well documented code, thorough documentation, and maybe even a user manual.

This could be something done within the university that I am at, as I will have more credibility as a student at the same institution.

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Rather than relying on a bunch of glued together scripts from 20 years ago, they have a modern analysis pipeline that they can rerun reliably and that will be standardized among other labs.

It's an illusion if you think that novel biology comes ready-made out of such pipelines. It's going back and forth with hands-on analysis. What can be automated is the preprocessing, but the actual downstream analysis, say starting from a count matrix, never will be. Preprocessing automation already exists, say nf-core or custom implementations. I use the same pipelines for preprocessing for years, it's always the same. But downstream never is.

It's always getting an idea from one analysis, validating, maybe re-doing, following up, and repeating. You might think it's different because of your, with respect, lack of real world experience, but there is a reason why basic research mainly operates on lots of custom scripts, because science and biology is so compex and noisy, therefore scrict automation in novel discoveries is so difficult, and often not remotely feasible.

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Thanks for the info.

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11 days ago

Don't freelance - it does not scale and you run the risk of selecting for mismanaged projects. What serious person would hire a freelancer for a scientific project?

Instead, redo the analyses found in interesting papers.

Build a portfolio of complete projects, and when someone asks you what you can do, you can show how you can associate genomic variants with body size for dog genomes, what diseases plagued Napoleon's army, and so on.

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I'm not sure how it works in other countries, but in the UK at least it would be quite unusual for a freelancer to be 'employed' on a research project where its is taxpayer grant money being used (unless maybe there was a fairly extensive track record of working with the instutition) - not impossible, but certainly uncommon and you'd need to be costed into the grant from the beginning usually.

Typically, you will need to jump through some hoops with a University to become an approved supplier, and I would hazard a guess that more often than not a research group which is looking to utilise this sort of help will already have access either to a collaborator or a centrally provided Uni resource.

That's not to say there isn't a need for this (my own academic lab did a lot of informatics with precious little dedicated support for this sort of thing), but whether there would be an engageable 'market' is another question.

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Not many hoops, Joe. There is not much red tape.

I have already worked 'freelance' and full-time with every major university in London plus many more across the UK, including Oxford and Cambridge.

Just be the best at what you do

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I'm not saying it doesn't happen - I am aware of a few instances myself, but I certainly consider it a bit of a rarety still (and the examples I know of were costed explicitly into the grant at the beginning so you could argue are more like Co-investigators or collaborators).

Unlike buying a reagent or something, most researchers/grants don't suddenly think "oh, I've just realised I need an informatician, where can I find one?".

You are certainly right that the instances where it does happen are by and large going to be where there is some pre-existing relationship and built trust.

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Hi Istvan,

Thank you for the realistic answer. I redid analyses from interesting papers by using both the workflows in your book, the Biostars handbook, and through the class I'm taking right now.

I don't think it's a bad idea to continue redoing analyses from published papers and I'm happy to go along that route, and I agree with the risks you pointed out in terms of running into mismanaged projects.

However, I'm wondering if there is a market need for people who need pipelines, and there may be labs out there who do not have access to a core or bioinformatics help in house.

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I think the most important thing is to have a visible portfolio - like a github repository that you can use to convince people that you can do the job.

That portfolio would be a good place to also explain your perspective on science, how you work, what you expect in return etc.

Just posting that you would like to work on a project is not enough. You need to demonstrate both competence and reliability. Most labs are risk-averse, they want evidence that you can take raw, messy data and deliver interpretable, publication-ready results without excessive supervision.

Once people can see clear examples of your work, engage where scientists actually look for help: here is a good place :-) , Slack groups for specific consortia, Twitter/X, Mastodon, or local genomics mailing lists.

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Yes, I have a github going of projects and analyses I did. The OP was more to do something productive over winter break, but there are plenty of other ways to build the portfolio - it was suggested to me to look at CellxGene datasets for single cell analysis.

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So where is your GitHub repo? - if you don't advertise it, how can anyone assess it?

Take a look at my profile - what do you see?

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I disagree. The trend is that many large companies and even universities are favouring freelance consultants over hiring of full- or fixed-time employees. Nobody wants to deal with the headache of pensions, trade-unions, and 'benefits' these days - the experienced freelance contractors are taking hold and standing ground.

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10 days ago
Kevin Blighe ★ 90k

I have been a successful freelancer for ~12 years.

Build connections, work with those who favour your growth and development, manage your social media profiles rigidly, and become the greatest expert ever known on Biostars :)

Simple.

Kind regards,

Kevin

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