Tutorial: Resources for flow cytometry bioinformatics
gravatar for Ahill
3.5 years ago by
United States
Ahill920 wrote:

The need for bioinformatics expertise in flow cytometry is increasing exponentially as the size and complexity of flow datasets grow.  I thought it would be worthwhile to post links to some tools and resources that may be beneficial for bioinformaticians getting starting with flow cytometry.  The list is highly biased and far from complete, so please feel free to add/comment.

Resources for flow cytometry bioinformatics

Community portals
ISAC http://isac-net.org/home.aspx
FlowCAP http://flowcap.flowsite.org

Data standards
FCS 3.1 http://isac-net.org/images/stories/documents/Standards/fcs3.1_normativespecification_20090813.pdf
Gating-ML http://flowcyt.sourceforge.net/gating
MIFlowCyt http://flowcyt.sourceforge.net/miflowcyt

Parsers, core data access
CFCS library http://sourceforge.net/projects/flowcyt
flowCore  http://bioconductor.org/packages/release/bioc/html/flowCore.html
Python FCM https://pythonhosted.org/fcm/

Unsupervised clustering/visualization
t-SNE https://lvdmaaten.github.io/tsne
VISNE http://www.c2b2.columbia.edu/danapeerlab/html/cyt.html
FLOCK http://sourceforge.net/projects/immportflock
SPADE http://www.bioconductor.org/packages/release/bioc/html/spade.html
CytoSPADE http://cytospade.org
Wanderlust http://www.c2b2.columbia.edu/danapeerlab/html/wanderlust.html
ACCENSE http://www.cellaccense.com

Semi/supervised analysis/visualization
FLAME http://software.broadinstitute.org/cancer/software/genepattern/FLAME
x-Cyt http://www.broadinstitute.org/mpg/xcyt
Citrus https://github.com/nolanlab/citrus

Other R packages
An ever-growing list, to get started go to:
http://bioconductor.org/help/workflows/highthroughputassays and scroll down to the "flow cytometry" section.
OpenCyto http://opencyto.org

Integrated applications
FlowJo http://www.flowjo.com
CytoBank http://cytobank.org
FCS Express https://www.denovosoftware.com
Kaluza http://www.beckman.com/coulter-flow-cytometry/software/kaluza-analysis

BD flow tool index http://www.bdbiosciences.com/research/multicolor/tools/index.jsp
GenePattern Flow Cytometry Suite

Mailing Lists
Purdue cytometry discussion list http://cyto.purdue.edu/hmarchiv

Data repositories

tutorial flow cytometry • 3.2k views
ADD COMMENTlink modified 14 days ago • written 3.5 years ago by Ahill920
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