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Devon Ryan
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Chico Fernandez
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Recent Replies
Comment: Question about a public scRNA dataset(single end)?
by
soda
•
0
I solved this problem with the help from a Senior Scientist(10x Genomics).
Thanks a lot!
----------
The senior scientist said:'
Since man…
Comment: can i take promoter upstream 2kb from tss ?
by
miles.thorburn
▴
140
Depending on which model system you are using it may also be worthwhile to check if any orthologs in better annotated species have promoter…
Comment: Why NB distribution is preferred in RNA-Seq while normal distribution is preferr
by
German.M.Demidov
★
2.7k
log-normal is less adequate. if e.g. our RNAseq is perfect, everything should be distributed according to Poisson and taking logarithm from…
Comment: Mirdeep not giving results.
by
Michael Dondrup
51k
The level of detail is still way too low. So you are running this in galaxy? Please provide more information or we cannot help you, includ…
Comment: Getting Uniprot IDs of a set of Genes
by
manaswwm
▴
250
From my previous visits to some protein entries on Uniprot, I was of the impression that some Uniprot entries are already linked to AlphaFo…
Comment: NCBI tblastx remote
by
lieven.sterck
13k
Would it be possible to put your whole post in English? thank you.
Comment: browser to visualize single cell data
by
lieven.sterck
13k
[kana][1] ?
[1]: https://github.com/jkanche/kana
Answer: How to convert strand specific RNASEQ bam to bigwig
by
a.bafna
•
0
Hi, thanks for reply. I indeed used "Only include reads originating from fragments from the forward or reverse strand" option and selected …
Comment: Issue with trend line and confidence interval
by
pdhrati02
▴
30
Hi Jeremy, Thank you for your suggestion, I tried it but it throws several warnings and error (x has insufficient unique values to support …
Comment: Cluster Profiler- enrichGO
by
Papyrus
★
2.1k
Personally I would use as universe, for both cases, all genes used as input for statistical testing.
Comment: With the X.M.P of my memories activated the Biostar screen takes about 40 second
by
Joe
20k
This is not a forum for BIOSTAR the motherboard manufacturer, you're asking in the wrong place.
Answer: Question about a public scRNA dataset(single end)?
by
ATpoint
60k
Seems indeed that R1 is missing, making the data basically useless. I would contact the authors.
Comment: With the X.M.P of my memories activated the Biostar screen takes about 40 second
by
Jean-Karim Heriche
25k
This post does not fit the theme of this forum.
Answer: Enrichment analysis and GSEA
by
jared.andrews07
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13k
GSEA stands for gene set enrichment analysis. It's not clear what you mean by "enrichment analysis" here. Regardless, GSEA at minimum needs…
Answer: can i take promoter upstream 2kb from tss ?
by
jared.andrews07
★
13k
Yes, that is a fairly common cutoff for defining a promoter region.
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