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updated 22 days ago by
Pierre Lindenbaum
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written 22 days ago by
Biostar
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Recent Replies
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
You're welcome. Yes, unfortunately you need quite a sample size for WGCNA simply because correlations with few samples are vastly underpowe…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
manuelmourato25
•
0
Ah, I see. So seen that what I want is the per-gene correlation, the minimum of 20/25 for the pearson correlation still applies.
Thank you …
Answer: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
I think you're misunderstanding some concepts here.
WGCNA is a framework that defines modules based on how and which genes are correlated …
Comment: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
GenoMax
142k
> most up-to-date build
Major build of the genome remains GRCh38. What you are looking at is the latest patch release for that build. Only…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
manuelmourato25
•
0
Thank you @atpoint . My apologies, maybe I should rephrase the question. I read the WGCNA docs already, and for the Pearson correlation tes…
Comment: How to extract cells of different species after mapping with combined genome?
by
GenoMax
142k
Can you confirm that this answer is specific for `singleron` technology and associated bioinformatics software.
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
What OS are you using? Are you able to run `blastn --help` and does it produce the in-line help?
> I used many query file.fasta
And tho…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
> However, even though 3 biological replicates per condition seems to be the norm in these experiments, many papers discuss that we need at…
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
ATpoint
82k
> I have curiosity, why do you make a desing with interceptor and without contrast matrix?
Because for a 2-group comparison you do not nee…
Comment: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
ATpoint
82k
If you have a one-click workflow ready and the necessary computation power to casually run it, then go for it. If it is any greater effort …
Comment: Seurat v5 and how to correctly integrate across multiple experiments
by
Papyrus
★
2.9k
In my experience integration methods are also often used for different samples/batches across the same technology. The crucial thing is to …
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
egascon
•
0
Thank you very much!
I have repeated the code adding arrayWeights() and it's works! In this case, I have a desing without intercept and c…
Comment: Is there any way to modify this pie chart ?
by
ohtang7
▴
40
Thank you, I've tried but I see only some appear in the chart in wrong segments.
Could you help me to solve this how ?
![enter image desc…
Comment: perseus software (version 1.5.5)
by
DGTool
▴
10
If the tool mentioned relates to the Perseus application from the Cox lab ([here][1]), it should be free to download after accepting the li…
Answer: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
DGTool
▴
10
At least so far to my knowledge, I don't think it would be worth re-mapping to GRCh38.p14, if it is too much trouble or with what you're lo…
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