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Comment: log2 fold change in RNA-seq analysis
Comment: log2 fold change in RNA-seq analysis
Answer: Ideal PC configurations and operating system for bioinformatics laboratory
correct way of analyzing cell proportions in singlecell data
Comment: How to interpret this plotMDS of three disease clusters?
gseGO: no term enriched under specific pvalueCutoff
Answer: Error with BiocParallel. No barcodes files found
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Recent Replies
Comment: log2 fold change in RNA-seq analysis
by
Hatchet
▴ 10
He means that the result of DEA will contain DEGs of A vs B. He gets LFC numbers in one column and positive LFC numbers will mean positive …
Comment: Z score
by
Akash D
▴ 50
I made this. Z scored on log2FC? will anyone support me on this? 3 groups A,B,C
Comment: log2 fold change in RNA-seq analysis
by
i.sudbery
19k
There is no such thing as a fold change of -2. Fold change is expression_in_condition_A/expresssion_in_condition_B as expression is a…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
DE with 100s of samples (particulalry if your experiemental design is more complex than 2 condition DE) can stretch consumer grade hardware…
Comment: Error with BiocParallel. No barcodes files found
by
NTerway
• 0
Thank you for your help! I will try this out. I am trying to implement a collaborator's pipeline on my data and they only use SCE, scran an…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
ATpoint
82k
Once you have the counts any n is possible on a regular analysis-grade laptop or workstation. Even hundreds of samples. It's really the pre…
Comment: Error with BiocParallel. No barcodes files found
by
ATpoint
82k
You just tell the function to remove the prefix for you. Not on the actual file. Then you can add this information to the SCE, like `sce$da…
Comment: log2 fold change in RNA-seq analysis
by
May Ling
• 0
Thank you for your response. I apologize if my understanding is incomplete. I would like to ask: if I have a fold change of -2, how can I c…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
Yeah, a standard 3x3 bulk experiement is very analysable on pretty much most good consumer laptops these days.
Comment: Error with BiocParallel. No barcodes files found
by
NTerway
• 0
Thanks for the explanation! If I remove the prefix (GSM3972009_69_) then I will lose the sample annotation and cannot track them. I am more…
Comment: Unexpected read length from NGS
by
ATpoint
82k
Seconding this. Just ask them.
Answer: Error with BiocParallel. No barcodes files found
by
ATpoint
82k
The function assumes simply barcodes.tsv(.gz), genes.tsv(.gz) and matrix.mtx(.gz), without additional pre- or suffixes by default. You can …
Comment: Free AI for R programming
by
GenoMax
142k
Try: https://ai.tinybio.cloud/
Comment: How to interpret this plotMDS of three disease clusters?
by
ATpoint
82k
arrayWeights is imo always a good idea with human (or generally large) cohorts. What you can also do is to use something like sva to estima…
Comment: Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Po
by
GenoMax
142k
Not what you want to hear but it is possible that the Ab data is not good quality as the cellranger message says (assuming everything ran p…
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