Question: what is the difference between single end and paired end in sequencing
4
gravatar for M K
5.9 years ago by
M K490
United States
M K490 wrote:

What is the main difference between single end and paired end in sequencing other than the first one used when we have a reference genome , and the second one in denovo case?

rna-seq • 51k views
ADD COMMENTlink modified 3.5 years ago by morteza.mahmoudisaber70 • written 5.9 years ago by M K490
2
gravatar for Sam
5.9 years ago by
Sam2.7k
New York
Sam2.7k wrote:

There are a lot of information regarding the topic on single end and paired end data. Generally, you can use paired end data even when you have reference data and you can also use single end reads for denovo assembly (as long as the read length is long with high coverage). 

A quick google search returns the following:

http://en.wikipedia.org/wiki/Paired-end_tag

ADD COMMENTlink written 5.9 years ago by Sam2.7k
1
gravatar for morteza.mahmoudisaber
3.5 years ago by
Japan
morteza.mahmoudisaber70 wrote:

This is short answer:

ADD COMMENTlink written 3.5 years ago by morteza.mahmoudisaber70
0
gravatar for ancient_learner
5.9 years ago by
India
ancient_learner620 wrote:

This thread from seq answers might be useful http://seqanswers.com/forums/showthread.php?t=503

ADD COMMENTlink written 5.9 years ago by ancient_learner620
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