what is the difference between single end and paired end in sequencing
3
5
Entering edit mode
10.1 years ago
M K ▴ 660

What is the main difference between single end and paired end in sequencing other than the first one used when we have a reference genome , and the second one in denovo case?

RNA-Seq • 58k views
ADD COMMENT
2
Entering edit mode
10.1 years ago
Sam ★ 4.7k

There are a lot of information regarding the topic on single end and paired end data. Generally, you can use paired end data even when you have reference data and you can also use single end reads for denovo assembly (as long as the read length is long with high coverage).

A quick google search returns the following:

http://en.wikipedia.org/wiki/Paired-end_tag

ADD COMMENT
1
Entering edit mode
7.6 years ago

This is short answer:

ADD COMMENT
0
Entering edit mode
10.1 years ago

This thread from seq answers might be useful http://seqanswers.com/forums/showthread.php?t=503

ADD COMMENT

Login before adding your answer.

Traffic: 3274 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6