Convert to Forward strand

Question

[win]

Hi all, is it possible to convert all VCF data to forward strand? Is there a script that can do that?

Thanks again in advance.

[Pierre Lindenbaum]

how did you get a VCF on the reverse strand ?

[Ashutosh Pandey]

lol

[Renesh]

The DP4 field under INFO contains the information for reads covering the strand for ref and alt bases. If you manipulate this field as per your requirement, you can get it.

Plz elaborate your question, so that we write the code for it.