Is the order of records in the bam file produced by bwa the same as the order in the input fastq file?

Question: Is the order of records in the bam file produced by bwa the same as the order in the input fastq file?

4.3 years ago by

Lina F • 150

Boston, MA

Lina F • 150 wrote:

Hi all,

I was wondering if the order of records in a bamfile produced by bwa aln/mem/sampe is guaranteed to be the same as in the fastq files that were used as input.

I checked the bwa manual but the only thing I found is this: "Repetitive read pairs will be placed randomly" (part of sampe description).

If anyone has an idea, I'd welcome your feedback!

Best,
~Lina

sampe bwa fastq mem aln • 2.2k views

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modified 4.3 years ago by lh3 ♦ 31k • written 4.3 years ago by Lina F • 150

4.3 years ago by

lh3 ♦ 31k

United States

lh3 ♦ 31k wrote:

Yes, the same.

ADD COMMENT • link written 4.3 years ago by lh3 ♦ 31k

4.3 years ago by

Renesh • 1.5k

United States

Renesh • 1.5k wrote:

Not necessarily. Sometime this can be in sorted in target mapping position. You can check this easily by command;

samtools view file.bam

ADD COMMENT • link written 4.3 years ago by Renesh • 1.5k

bwa won't coordinate-sort for you. It's possible that the default output order matches the input. One would just need to compare an input and output to see. If it doesn't and Lina F needs that, then bowtie2 with the --reorder option will work.

ADD REPLY • link written 4.3 years ago by Devon Ryan ♦ 88k

I apologize for any mistaken assumption.

ADD REPLY • link written 4.3 years ago by Renesh • 1.5k

No worries, lh3 just replied anyway and he's obviously the definitive source on this sort of thing :)

ADD REPLY • link written 4.3 years ago by Devon Ryan ♦ 88k

4.3 years ago by

Lina F • 150

Boston, MA

Lina F • 150 wrote:

Thanks for the feedback!

It looks like the default order matches the input for my data, but I just wanted to find out if that is guaranteed by bwa. To be on the safe side, I'll assume it's not guaranteed.

ADD COMMENT • link written 4.3 years ago by Lina F • 150

A wild guess without having checked anything...: If bwa runs on one thread then output order = input order. If bwa runs on multiple threads input and output orders might differ.

ADD REPLY • link written 4.3 years ago by dariober ♦ 9.9k

sounds reasonable! Currently I'm running bwa on a single thread but I might have to move to more than one in the future...

ADD REPLY • link written 4.3 years ago by Lina F • 150

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