I am new to this forum. I am interested in SS-RNAseq and would like to know what statistical method should be used to calculate the number of cells (minimum) needed to sequence per condition.
In a hypothetical experiment, a study needs to compare DE in population of epithelial cells infected with papilloma virus versus uninfected epithelial cells isolated from the same individual. Single cells are collected for analysis.
How many cells are needed? And how can that be calculated?
It seems that existing published literature on ssRNA-seq does not provide much details on how they derived their sample size but can range from as little as 25 to few hundreds.
Is edgeR RNASeqPower be a suitable tool for this purpose?