Entering edit mode
8.2 years ago
mahnazkiani ▴ 50
We are trying to do RNA-seq for mitochondrial and nuclear RNA and we sent the sample to genomics center, they made the library and they found a prominent peak in all of sample around 410bp. Any guess about that?
My samples are from plant
Sounds like you have some RNA that is 410bp. What was the question?
And how is this bioinformatics? Expecting the question to close soon because it's not a technology question, but a wetlab question.
I'll leave this open for the time being (though I expect someone else will close it) simply to not dissuade someone who happens to have good insight from replying. However, I suspect you'll get better feedback from SEQanswers. The regulars over there tend to bend more toward the wet-lab side of the spectrum than those here.
BTW, it's somewhat important to know at what point in the library prep this was noticed. There are a bunch of rRNAs in plants, so they can show more peaks than you'd see in mammals (presumably that's what the genomics center is used to seeing). If/when you post on SEQanswers, you might attach the whole plot and provide these details.