Question: Constructing the ancestral state of amino acid
0
gravatar for RT
4.5 years ago by
RT340
European Union
RT340 wrote:

Hello Everyone,

I have the results from codeml analysis where I have identified few sites that are under positive selection in my target species. I am now looking for the change in amino acid in the nearest species and the ancestral amino acid. I have around 20 such genes where I have identified positive selection in orthologs of these genes. I can easily identify the amino acid in nearest species from the MSA of these genes. I am wondering if it is fair enough to identify the ancestral amino acid same way- looking at MSA for amino acid in the distant species retained in the orthology cluster. or there is any other way to construct the ancestral state of these sites?

I hope I am clear in asking the question.

Thanks.

selection codeml paml • 1.2k views
ADD COMMENTlink modified 4.4 years ago by Michael Dondrup46k • written 4.5 years ago by RT340
1
gravatar for Juke-34
4.4 years ago by
Juke-342.8k
Sweden
Juke-342.8k wrote:

You could try from the DNA sequence using the Ortheus tool. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577868/). It allows to reconstruct the ancestral state of DNA sequences aligned. 

ADD COMMENTlink modified 4.4 years ago • written 4.4 years ago by Juke-342.8k
1
gravatar for Michael Dondrup
4.4 years ago by
Bergen, Norway
Michael Dondrup46k wrote:

FastML http://fastml.tau.ac.il/http://nar.oxfordjournals.org/content/40/W1/W580.long is a web server for ancestral sequence reconstruction, using the sequence MSA plus phylogenetic tree as input. I don't know if that answers your question, but I have seen papers that use both PAML and FastML and compare the results.

ADD COMMENTlink written 4.4 years ago by Michael Dondrup46k
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