7.5 years ago by
In addition to what has been said here already, remember the folowing:
Nobody said that all biological replicates must be performed at the same time. In fact, you can add the biological replicates now. If your lab invests just little bit more in replication, repeating the experiment and adding two more biological replicates, your data will become so much more valuable.
The added value is so relevant in this case, from almost unusable and not publishable (anymore), to a analysis allowing for assessing significance!
Think about it: instead of helping the experimenter to salvage their crappy experimental design (and that's what I would seriously call it), resist the temptation and ask them to improve their experiment instead. You will avoid wasting a lot of time, yours and the experimenters (especially, unfortunately many don't understand the statistical implications).