Question: Microrna Alignment And Output
2
gravatar for Hamilton
9.2 years ago by
Hamilton290
Hamilton290 wrote:

Post alignment of microRNA and mapping against Mirbase I have a column ID with the precursor as well as the mature microRNA name, on parsing the column I have the list with the mature microRNA which has duplicates with different read counts. Which one of the microRNA should I consider for the the downstream analysis as some of the names are in duplicate. Please suggest.

ADD COMMENTlink modified 4.6 years ago by Biostar ♦♦ 20 • written 9.2 years ago by Hamilton290

duplicate because they are the same mature sequence from different species?

ADD REPLYlink written 9.2 years ago by Jeremy Leipzig19k

nope they are all same species. mmu all mouse.

ADD REPLYlink written 9.2 years ago by Hamilton290
0
gravatar for Rm
9.2 years ago by
Rm8.0k
Danville, PA
Rm8.0k wrote:

Refer to this post.

My two cents: Same mature miRNA with different precursors as they map to different chromosomal locations. I guess treat them as separate with same counts, unless you are interested in a miRNA of a specific location.

ADD COMMENTlink modified 17 months ago by Ram32k • written 9.2 years ago by Rm8.0k
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